In
this experiment, students isolate their own genomic DNA from cheek cells. They
then use polymerase chain reaction (PCR) to amplify (make millions of copies of)
a region of chromosome 16 that may or may not contain a ėjumping gene.î Finally,
students use agarose gel electrophoresis to separate the DNA according to size.
Pieces of DNA without the ėjumping geneî would be 550 bases long, while DNA containing
the ėjumping geneî would have been 850 bases long.
DNA Sequencing
BIO/CHEM 375 -- Molecular Biology Laboratory
In
this class, students use the ABI PRISM 377 DNA Sequencer to determine the exact
nucleotide sequence of a segment of DNA (e.g., a plasmid). This is achieved by
combining the sequence of multiple samples and then performing a computer-assisted
alignment with software.
Rockfish Species Identification
BIO 427 -- Fisheries Science and Conservation
In
this class, students use Restriction Fragment
Length Polymorphism (RFLP) analysis to distinguish
the planktonic stages of various rockfish species.
First, students isolate genomic DNA from fin tissue.
The DNA is then amplified by PCR and digested
by a restriction endonuclease. Finally, the fragments
are analyzed by agarose gel electrophoresis
Black-BRCA2
Mutant R2520X Allele Screen (Download
powerpoint poster 1.8MB)
The BRCA2 breast cancer gene is
a tumor suppressor gene that acts as both a transcription
factor and as part of the cells DNA repair system.
Disruption of this gene may lead to the development
of breast, ovarian, prostate, colon and/or pancreatic
cancer. The following experiment deals with a
specific mutation within the BRCA2 gene located
at amino acid 2520 of 3418. The R2520X mutation
has been classified as a family-inherited factor,
which resides on my (R.Kloch) maternal side of
the family. The purpose of the following experiment
was to run an allelic screening test using my
own DNA to determine if I am a positive carrier
of the R2520X allele.
Steinmaus
"Genetically Modified Organisms: Who is growing
them? Do They Move?" 7.1Mb
word Doc download
Herbicide
Resistance: Crop vs. Weed1.1Mb
word Doc download
Steps
To Development of Classroom Exercise
- Faculty
shows interest for course experiment and contact
UBL
- Director
consults with faculty to determine exercise
objectives and protocols
- Protocols
usually adapted from journal articles
-
UBL technicians adapt to class setting and optimize,
troubleshoot and then train faculty or students.
